IGFBP7：免疫治疗新靶点

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2026/6/5 6:07:40

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前言胰岛素样生长因子结合蛋白7IGFBP7是胰岛素超家族的生长促进肽成员可与胰岛素和IGF结合调控细胞生长和分化。IGFBP7在不同的肿瘤类型中表现出抑制或促进肿瘤生长的“自相矛盾”活性。研究发现IGFBP7可增强治疗性单克隆抗体的抗肿瘤作用并在某些癌症血清中含量高正在成为多种疾病诊断的生物标志物。01 IGFBP7的生理学和病理学意义IGFBP7在多种组织中表达包括大脑、肝脏、胰腺和骨骼肌。IGFBP7通过调节组织中IGF的生物利用度和其受体INSR-A、INSR-B、IGF-1R、IGF-2R的结合来发挥功能。通过与胰岛素或IGF结合激活下游三个重要的信号转导通路IRS-PI3K-AKT-mTOR、Ras-MEK-ERK和Ras-MAPK诱导蛋白合成、细胞增殖、生长维持及抗凋亡。IGFBP7在IGF依赖型或IGF独立型癌症类型中表达有所差异。在IGF独立型癌症中IGFBP7可促进肿瘤细胞生长、血管生成、上皮间质转化(EMT)和细胞凋亡。IGFBP7还在多种类型的癌症中呈现肿瘤抑制活性。通过与CD93结合IGFBP7破坏肿瘤血管生成并增强免疫浸润进而增强CD93单克隆抗体的免疫治疗效果。随着研究的深入IGFBP7正在成为各种疾病的生物标志物包括急性肾损伤、心衰和癌症。IGFBP7和癌症相关的潜在机制和途径。源自https://doi.org/10.3389/fonc.2020.0072702 IGFBP7从实验室到临床应用IGFBP7在胃癌、前列腺癌、结直肠癌、膀胱癌和食道癌中表达上调。IGFBP7在肿瘤生成中的复杂生物学作用和分子机制仍然是临床前关注的焦点。截至2023年8月临床研究主要集中在IGFBP7在急性肾损伤和心衰中的作用。03 IGFBP7在研究中的应用IGFBP7蛋白和抗体用于研究肿瘤微环境和配受体相互作用机制。Sun团队使用IGFBP7抗体货号13100-R003义翘神州和CD93抗体货号12589-MM01义翘神州阻断IGFBP7和CD93相互作用。通过免疫荧光染色检测组织样本中IGFBP7的表达情况。Yamamoto团队利用重组IGFBP7蛋白货号13100-H08H义翘神州研究LRP1的配体。用于流式细胞术检测添加人源CD93单抗货号12589-MM01义翘神州或IGFBP7单抗货号13100-R003义翘神州可显著降低IGFBP7蛋白与CD93-HEK293T细胞的结合研究认定IGFBP7是CD93的配体。对照组为野生型HEK 293T细胞红色实验组为转染CD93的HEK293T细胞。源自doi: 10.1126/scitranslmed.abc8922用于免疫荧光检测对来自正常组织的胰腺、皮肤和原位KPC、皮下植入B16的肿瘤样本进行染色结果发现IGFBP7在肿瘤脉管系统中表达上调。IGFBP7绿色和CD31红色。源自doi: 10.1126/scitranslmed.abc8922用于LC-MS/MS质谱检测评估了不同浓度下HMGB1货号10326-H08H义翘神州、IGFBP7货号13100-H08H义翘神州、SPARC货号10929-H08H义翘神州、LIF货号14890-H08H义翘神州等重组蛋白与LRP1之间的结合亲和力结果发现HMGB16.4nM、IGFBP711nM、SPARC41nM等亲和力较高LIF200nM亲和力较低。源自doi: 10.1016/j.matbio.2022.08.007✦IGFBP7明星产品IGFBP7 Protein, Human, Recombinant (hFc AVI Tag), Biotinylated, HPLC-verified, Cat: 13100-H41H-B高纯度Purity ≥ 90 % as determined by SDS-PAGE and SEC-HPLC结合活性Immobilized human CD93 protein can bind IGFBP7 protein. The EC50 is 7-21 ng/mL.IGFBP7 Protein, Human, Recombinant (hFc AVI Tag), Biotinylated, HPLC-verified, Cat: 13100-H41H-B结合活性Immobilized human IGFBP7 Protein can bind human CD93 protein with a linear range of 0.7-5.0 μg/mL.【参考文献】1. Qiaoyun Zhao, et al. Increased IGFBP7 Expression Correlates with Poor Prognosis and Immune Infiltration in Gastric Cancer. Journal of Cancer 2021. doi: 10.7150/jca.50370.2. Yi X, et al. IGFBP7 and the Tumor Immune Landscape: A Novel Target for Immunotherapy in Bladder Cancer. Front. Immunol. 2022. doi: 10.3389/fimmu.2022.898493.3. CD93 Blockade Stabilizes Tumor Vasculature to Improve Therapy Response. Cancer Discovery (2021) 11(10):2368. doi: 10.1158/2159-8290.Cd-rw2021-113.4. Huang X, et al. The Diagnostic Value of Serum IGFBP7 in Patients with Esophageal Squamous Cell Carcinoma. J Cancer 2019, 10(12):2687-2693.5. Jin L, et al. Insulin Growth Factor Binding Protein 7 (IGFBP7)-Related Cancer and IGFBP3 and IGFBP7 Crosstalk. 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